No4(3) 2020
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DOI 10.37219/2528-8253-2020-4-55 |
Melnykov O.F., Peleshenko N.A., Timchenko M.D., Rylska O.G.,
Prilutskaya A.D.,
Bredun A.Yu., Timchenko S.V., Bil B.N.
Anti-inflammatory mechanisms in the action of the antiseptic drug lizak
(clinical and immunological study) |
Melnikov Oleg F.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine.
Head of the Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Doctor of Medical Sciences, Professor
E-mail: elenamelnykova@gmail.com
ORCID ID: https://orcid.org/0000-0002-1261-8647
https://www.scopus.com/authid/detail.uri?authorId=7006385903
https://www.scopus.com/authid/detail.uri?authorId=7003452154
Peleshenko Natalia O.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine
Department of Inflammatory Diseases of ENT Organs
Senior Researcher
E-mail: peleshenkon@gmail.com
Timchenko Мarina D.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine.
Senior Researcher
Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
PhD
E-mail: svtim@i.ua
Rylska Oksana G.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine
Department of Inflammatory Diseases of ENT Organs
Senior Researcher
Email: oxanar@ua.fm
Bredun Alexander Yu
National Medical Academy of Post-Graduate Education Named After P.L. Shupik
Department of Pediatric Otorhinolaryngology, Audiology, Phoniatrics
Associate Professor, PhD.
E-mail: abredun@ukr.net
Timchenko Sergei V.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine.
Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Leading Researcher
Candidate of Medical Sciences
E-mail: svtim@i.ua
Bil Bogdan Nazarovich
Chief otolaryngologist in Kiev, head of the State Scientific and Practical
Center for Endoscopic Rhinolaryngology and the City Phoniatric Center, Head
of the Otolaryngology Department of the Kiev City Clinical Hospital No. 9,
Candidate of Medical Sciences
Associate Professor, Department of Otolaryngology, National Medical Academy
of Postgraduate Education named after P.L. Shupika |
Abstract
Topicality: In recent years, ideas about the mechanisms of action of
a local lysozyme containing antiseptic have been significantly expanded.
Data on the availability of antiviral drugs and a positive effect on
regeneration processes have been obtained.
Objective: In order to further study the properties of the drug, its
effect on the mechanisms of inflammation in a clinical setting and model of
inflammation in an animal experiment was investigated.
Material and methods: Clinical and immunological studies were
conducted in 22 children aged 9-12 years, of which 10 people represented a
group of healthy donors. 12 people with exacerbation of chronic catarrhal
pharyngitis received the drug Lizak in the form of monotherapy according to
the instructions.
The oropharyngeal secretion was studied, where the pro-inflammatory cytokine
interleukin-1β (Cytokine, RF), lactoferrin (Vector Best, RF), as well as the
macrophage inflammatory protein MIP-1b (Hucaltbiotecknolgy, Netherlands)
were determined by the ELISA method.
As an analyzer, a Lab line reader (Austria) was used. The studies were
carried out at two points: before the start of therapy and a day after its
completion. Under the experimental conditions, a model of caraginan edema of
rat paws was used, which, prior to the introduction of phlogogen, received
per os drug lizak.
The degree of edema was determined using a special installation with an
installed micrometer for measuring the thickness of the feet in the time
interval from 0 to 24 hours. In addition, the levels of proinflammatory
cytokine interleukin-1β and anti-inflammatory interleukin-10 were determined
in the blood serum of animals at the same time intervals. Statistics were
carried out using the ‘t’ –Student criterion.
Results: Under monotherapy with the drug Lysak in children with acute
pharyngitis, a decrease in the content of proinflammatory factors,
interleukin-1β and Mip-1b, in the oropharyngeal secretion is shown. When
studying the effect of the drug on the development of aseptic inflammation
(stage of exudation) in the foot of rats, it was found that the drug Lizak
had an inhibitory effect on the development of edema in the range of 1-4
hours, after which its effect was not detected (24 hours).
In the control group and the “pure” control group, inflammatory edema was
detected compared to the initial level (p=0.02) at 4 hours and was not
detected after 24 hours. The drug Lysac has the ability to inhibit the
development of aseptic local inflammation in the initial stages of its
development. Confirmation of the systemic nature of the action of the drug
is to reduce the level of pro-inflammatory cytokine-interleukin-1 in the
blood serum of animals by 4 hours from the onset of the inflammatory
process.
Conclusions: The drug Lizak actively reduces the levels of
pro-inflammatory cytokines both systemically and with local use and has a
pronounced decongestant effect. |
Keywords
lysozyme containing antiseptic, inflammation, cytokines, model of edema in
the experiment. |
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